The girl, a student of Fort Myers High School, Florida, would, according to reports, stay in the boys' toilets after school hours.
The incident was reportedly filmed on Snapchat, causing rumours to start making rounds in the school, till the authorities caught wind of it.
CCTV images have now seemingly confirmed the rumours, as videos show up to 25 boys going in and out of the toilets during that time.
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Speaking with Fox News, student Petislin Cadeau said: "Like, how did it happen? Like, where was everybody at?"
Cadeau, who said there were rumours that the football team was involved, added: "I heard a video was around but I never really saw it or anything. It just like circulated around."
According to a spokesman for the school district, no arrest has been made just yet, as the the incident is still being dealt with internally.