"Doing the sums we arrive quickly at half a billion euros... these are funds that will be available from Monday," Autostrade per l'Italia chief executive Giovanni Castellucci said at a press conference.
Castellucci said that Autostrade, which runs almost half OF Italy's motorway network, would be able to build a new steel bridge in "eight months".
Shares in Atlantia, Autostrade's holding company, plummeted last week after the government said it would seek to revoke its lucrative motorway contracts.
Castellucci also said that a fund worth "millons" would be given to Genoa city council for the families of the victims of the disaster, which has killed at least 39 people.
Another fund worth "tens of million" would be given to the city to help relocate the hundreds of people evacuated from their homes following the bridge's collapse on Tuesday.
Autostrade chairman Fabio Cerchiai said Castellucci would remain CEO despite government calls for the company's management to step down.