The group said seven million Nigerian youths would force Buhari out of office if he fails to resign.
The group gave the President an eight-week ultimatum to step down or face forceful removal by seven million Nigerian youths in 22 states.
Buhari returned to the United Kingdom for medical follow-up on May 7, 2017. He had spent 49 days in London on his first trip.
The youth group's chairman, Seriki Olorunwa, said the prevailing health condition of the president had made it impossible for him to govern the country.
"We urge the Senate, House of Representatives, state house of assemblies and parastatals to persuade Mr. President to resign and hand over to an interim president in the interest of Nigerians," Olorunwa said.
He noted that Nigerian youths are ready to fight than to live as slaves, but assured that they would not shed blood.
The group lamented the spate of killing, kidnapping, and robbery in the country, saying the All Progressives Congress (APC) has subjected Nigerians to humiliation because of its self-serving activities.