Serena was scheduled to face Sharapova at the Court Philippe Chatrier on Monday, June 4 but instead called for a press conference where she announced that she will be pulling out.
"Unfortunately I have been having issues with my pec muscle, right now I can't actually serve," she said.
"The first time I felt it was against (Julia) Goerges (third round), it was really painful. In my doubles yesterday I tried lots of different tapings. It didn't really get a lot better."
''I'm going to get an MRI tomorrow. I won't know until I get the results. I'm beyond disappointed. I gave up so much, time with my daughter, all for this moment, so it's really difficult to be in this situation. I always try to think positive."
The 36-year-old on Sunday, June 3 played a three set doubles match alongside sister Venus which they ended up losing 6-0.