A second crash in as many days has forced pre-race favourite Nairo Quintana out of this year's Vuelta a Espana due to a shoulder injury.
Quintana " winner of the Giro d'Italia earlier this year " led the event going into Tuesday's stage 10.
The Movistar rider, who did not compete in this year's Tour de France, went over his handlebars during the 36.7km individual time trial and did not get back on his bike for almost two minutes.
He then lost further time before the stage ended, with Alberto Contador (Saxo-Tinkoff) taking the race lead as a result.
The Colombian was three minutes and 28 seconds behind Contador when the race resumed on Wednesday, ending his realistic chances of victory, but the 24-year-old expressed a desire to ride on and support team-mate Alejandro Valverde, who is second in the general classification standings.
But another accident forced Quintana to abandon his plans after he crashed 20 kilometres into Wednesday's 153.4km stage from Pamplona to San Miguel de Aralar.
Movistar posted on their official Facebook page: "Nairo Quintana has been forced to abandon the Vuelta a Espana after suffering a new fall around the 20-mile mark in today's stage."
Quintana fell on his right shoulder and was taken away by an ambulance for tests in Pamplona, which revealed a fractured shoulder blade.
A Movistar statement read: "Confirmation: @NairoQuinCo has suffered a displaced fracture in his right-hand scapula, will undergo surgery on Thursday (10am)."