Defending champions Chile have been dealt an early blow after defender Eugenio Mena was ruled out for the remainder of the Copa America Centenario due to injury.
Mena suffered a torn right hamstring during Monday's 2-1 loss to 2015 runners-up Argentina in Santa Clara, California, with CONMEBOL confirming a replacement cannot be called up as the 27-year-old already played in the centenary tournament.
The 44-cap veteran was taken from the field nine minutes into the second half.
Chile, who have lost five of their past six matches, face Bolivia in a must-win Group D clash on Friday.
Juan Antonio Pizzi's Chile then close out the group stage with a fixture against Panama on June 14.