Head coach Jorge Sampaoli selected his 23 players to represent the country at the World Cup with one major surprise exclusion Mauro Icardi who finished the season as the Italian Serie A joint top scorer with 29 goals.
Preferred ahead of Icardi are Juventus duo of Gonzalo Higuain and Paulo Dybala and recovering Manchester City striker Sergio Aguero.
Only eight members of the 23 players selected participated at the 2014 FIFA World Cup in Brazil where Argentina finished as runners-up.
Argentina are drawn in group D of the World Cup alongside Iceland, Nigeria and Croatia.
Speaking at a press conference after unveiling the list Sampaoli defended his choice of players.
He said, "We came to these names after a lot of analysis in each position.
"I tried to be as fair as possible in relation to the game i wanted. There are young players with a lot of potential. It will be important to move experienced players."
Argentina's World Cup squad
Goalkeepers: Sergio Romero (Manchester United), Willy Caballero (Manchester City), Franco Armani (River Plate).
Defenders: Gabriel Mercado (Sevilla), Cristian Ansaldi (Torino), Nicolas Otamendi (Man City), Federico Fazio (Roma), Marcos Rojo (Man United), Nicolas Tagliafico (Ajax), Marcos Acuna (Sporting), Eduardo Salvio (Benfica).
Midfielders: Javier Mascherano (Hebei), Lucas Biglia (Milan), Ever Banega (Sevilla), Giovani Lo Celso (PSG), Manuel Lanzini (West Ham), Angel Di Maria (PSG), Maxi Meza (Independiente).
Forwards: Lionel Messi (Barcelona), Paulo Dybala (Juventus), Sergio Aguero (Man City), Gonzalo Higuain (Juventus), Cristian Pavon (Boca Juniors).