Jordan Spieth and Patrick Reed will aim to lead a Ryder Cup fightback from the United States in Sunday's decisive singles matches.
The rookie pair were unbeaten through the first two days at Gleneagles and only denied three wins from three when Justin Rose holed out to secure a half-point alongside Martin Kaymer in Saturday's foursomes.
That helped Europe take a 10-6 lead into Sunday's singles action, the same advantage the USA held at Medinah two years ago before they were sensationally overhauled.
Spieth will take on Graeme McDowell first up before Reed takes on Sweden's Henrik Stenson.
The third tie of the day will see Rory McIlroy face Rickie Fowler - the American having run the world number one close at The Open in July.
McIlroy picked up his first win of this year's competition in Saturday's second session alongside Sergio Garcia and the Spaniard is set to take on Jim Furyk for a second successive Ryder Cup.
Furyk fell to a one-hole defeat at Medinah, with the loss proving a turning point as Europe fought back to clinch victory.
Ian Poulter will meet Webb Simpson, who has not featured since enduring a difficult first morning's play on Friday, while the in-form Rose will look to complete an unbeaten campaign against Hunter Mahan.
Kaymer - the man who ensured Europe retained the Ryder Cup at Medinah - will hope to create further positive memories against Bubba Watson.
Thomas Bjorn comes up against Matt Kuchar, while rookies will be involved in the final two games, with America's Jimmy Walker playing Lee Westwood and Victor Dubuisson, another unbeaten European, last out against Zach Johnson.
Sunday's other matches will see home favourite Stephen Gallacher do battle with Phil Mickelson and Jamie Donaldson challenge Keegan Bradley.
Singles draw in full: Graeme McDowell v Jordan Spieth, Henrik Stenson v Patrick Reed, Rory McIlroy v Rickie Fowler, Justin Rose v Hunter Mahan, Stephen Gallacher v Phil Mickelson, Martin Kaymer v Bubba Watson, Thomas Bjorn v Matt Kuchar, Sergio Garcia v Jim Furyk, Ian Poulter v Webb Simpson, Jamie Donaldson v Keegan Bradley, Lee Westwood v Jimmy Walker, Victor Dubuisson v Zach Johnson.