Andrew and David Fifita will not be available to play for Cronulla Sharks while an investigation into their alleged conduct is ongoing.
Cronulla Sharks have stood down Andrew and David Fifita amid allegations the twins were involved in a foul-mouthed confrontation with a junior referee during a game in Penrith on Saturday.
The twins were in the spotlight this weekend after claims that they physically and verbally abused an official at Glenwood.
Sharks CEO Lyall Gorman on Sunday expressed his "great concern" over the claims, and warned the club have a "zero tolerance" policy on such behaviour.
Andrew featured Sunday's 18-16 NRL win over Canterbury Bulldogs, but his brother David did not feature due to injury.
The Sharks on Monday revealed that the duo will now not be considered for selection while the matter is investigated.
A statement from the Sharks said: "The Club advises that Andrew and David Fifita have been stood down pending further investigation and to allow them and their representatives to fully address matters relating to allegations of inappropriate behaviour at an A Grade Men's match under the jurisdiction of the Penrith and District Junior Rugby League.
"The Club has today received, and is reviewing and assessing, statements from a variety of people present at the match including match officials, participating club representatives, Ground officials and independent witnesses. In assessing these reports it is evident that conflicting versions of events exist.
"There is a process which must now be allowed to occur to give the players the opportunity to review that information and respond and that process is underway.
"As a part of that process the players have advised they intend to defend the more serious charges levelled against them.
"The Club has been advised that the previously reported video evidence of the alleged incident does not exist. The players' legal representatives have expressed their frustration in this regard as they were looking to use this evidence to support their case."