Jim Furyk says having good team chemistry is vital if the United States want to claim a first Ryder Cup win since 2008.
Europe have won the past two events, with the US last tasting success at Vahalla back in 2008.
A superb comeback from Europe at Medinah in 2012 saw them claw a four-point deficit back on Sunday to win by one point, with Furyk losing his singles match to Sergio Garcia.
The US have not won on European soil since 1993, and Furyk stressed the importance of the visitors working well together if they want to end that disappointing run at Gleneagles this week.
"Chemistry is extremely important," he told a news conference on Tuesday. "Not just how the players mix together as a 12 but also, more importantly perhaps, how they play together.
"I'm sure [captain] Tom [Watson] will do a wonderful job and provide the players with the best chance of doing well."
The 44-year-old, preparing for his ninth Ryder Cup appearance, spent Tuesday getting to know the Scottish course alongside team-mates Zach Johnson, Hunter Mahan and Patrick Reed.
Following that round he said his own confidence was high after six top 10 finishes in the last eight tournaments coming into the Ryder Cup.
"I've had a good stretch since the British Open," he added. "I've got a lot of confidence in my game - I feel good.
"I'm slowly starting to get over the jet-lag - I certainly feel better with a golf club in my hand than I did yesterday and just really trying to get ready."