Gyan picked up an injury while training with his club and is not expected to recover in time for the World Cup qualifier.
The Black Stars will travel to face Egypt in the matchday 2 of the qualifiers on Sunday, November 13.
Gyan picked up an injury while training with his club on Tuesday, November 1 and the 30-year-old is not expected to recover in time for the World Cup qualifier.
Ghana are second in Group E with just a point following their goalless home draw with Uganda in the opening round of the qualifiers.
Egypt lead the group with three points after their away win at Congo.
The leader of the group qualifies for the World Cup.
Ghana squad to face Egypt
Goalkeepers: Razak Braimah (Cordoba, Spain), Adam Kwarasey (Rosenborg, Norway) Richard Ofori (Wa All Stars)
Defenders: Harrison Afful (Columbus Crew, USA), Andy Yiadom (Barnsley, England), Baba Rahman (Schalke 04, Germany), Daniel Amartey, Jeffery Schlupp (Lesicester City), John Boye (Sivasspor, Turkey), Jonathan Mensah (Anzhi, Russia), Edwin Gyimah (Orlando Pirates (South Africa)
Midfielders: Agyemang-Badu (Udinese, Italy), Afriyie Acquah (Torino, Italy), Thomas Partey (Athletico Madrid, Spain), Mubarak Wakaso (Panathinaikos, Greece), Frank Acheampong (Anderlecht, Belgium), Christian Atsu (Newcastle United, England), Alhassan Wakaso (Rio Ave, Portugal), Samuel Tetteh (Liefering, Austria)
Forwards: Jordan Ayew (Aston Villa, England), Abdul-Majeed Waris (Lorient, France), Andre Ayew (West Ham, England), Ebenezer Assifuah (FC Sion, Switzerland)